Quantitation of Membrane Glycoprotein lila on Intact Human Platelets Using the Monoclonal Antibody, AP-3
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چکیده
A murine monoclonal antibody specific for glycoprotein (GP)llla was prepared by immunization with a GPIIband GPIIla-enriched Triton X-1 14 extract of platelet membranes. This antibody, designated AP-3, was shown by indirect immunoprecipitation to react solely with GPIIIa derived from either P1 Alpositive or -negative individuals. The epitope on GPIIIa recognized by AP-3 is expressed on dissociated GPIIIa as well as on Ca 2-dependent complexes of GPIIb and GPIIIa, as shown by crossed immunoelectrophoresis in the presence or absence of EDTA. A previously described monoclonal antibody specific for the GPIIb/llla complex (AP-2) inhibited platelet aggregation induced by ADP, thrombin, collagen, or arachidonic acid (Pidard et al. J
منابع مشابه
Quantitation of membrane glycoprotein IIIa on intact human platelets using the monoclonal antibody, AP-3.
A murine monoclonal antibody specific for glycoprotein (GP)IIIa was prepared by immunization with a GPIIb- and GPIIIa-enriched Triton X-114 extract of platelet membranes. This antibody, designated AP-3, was shown by indirect immunoprecipitation to react solely with GPIIIa derived from either P1A1-positive or -negative individuals. The epitope on GPIIIa recognized by AP-3 is expressed on dissoci...
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